Jurnal Internasional PRMT5 berinteraksi dengan onkoprotein BCL6 dan diperlukan untuk pembentukan pusat germinal dan kelangsungan hidup sel limfoma.
The germinal center (GC) reaction plays an important role in producing humoral immunity and is believed to cause most cell B lymphomas. Entry and exit GC is a strictly regulated process, controlled by the actions of transcription factors such as BCL6. Here, we show that the arginine methyltransferase 5 (PRMT5) protein, symmetry of dimethyl arginine methyltransferase, is also needed for GC formation and affinity maturation. PRMT5 contributes to GC formation and affinity maturation at least in part through direct interaction with and methylation of BCL6 in arginine 305 (R305), a modification needed for the repressive effects of full BCL6 transcriptional. Inhibition of PRMT5 in B cell lymphoma cells causes a significant regulatory increase of the BCL6 target gene, and concomitant inhibitions of both BCL6 and PRMT5 show synergistic killing of BCL6-express lymphoma cells. Our study identified PRMT5 as a new regulator of GC reactions and highlighted the mechanistic thinking of cotargeting PRMT5 and BCL6 in lymphoma.